Anandamide Work As Antidepressant

Boosting the amounts of a marijuana-like brain transmitter called anandamide produces antidepressant effects in test rats, according to American and Italian researchers.

The researchers, led by Daniele Piomelli, the Louise Turner Arnold Chair in Neurosciences and director of the Center for Drug Discovery at the University of California, Irvine, used a drug they created, called URB597, which blocks anandamide degradation in the brain, thereby increasing the levels of this chemical.

“These findings raise the hope that the mood-elevating properties of marijuana can be harnessed to treat depression,” Piomelli said. “Marijuana itself has shown no clinical use for depression. However, specific drugs that amplify the actions of natural marijuana-like transmitters in the brain are showing great promise.”

The researchers administered URB597 to chronically-stressed rats that demonstrated behaviors similar to those seen in depressed human patients. The stressed rats treated with the drug, after five weeks of treatment, were behaving similarly to a comparison group of unstressed animals.

URB597 works by inhibiting an enzyme in the body FAAH that breaks down anandamide. Anandamide, dubbed as “the bliss molecule” for its similarities to the active ingredient in marijuana is a neurotransmitter that is part of the brain’s endocannabinoid system. In studies by Piomelli and others, anandamide has been shown to play analgesic, anti-anxiety, and antidepressant roles besides being involved in the regulation of feeding and obesity. Inhibiting FAAH activity boosts the effects of anandamide without producing the “high” seen with marijuana.

Piomelli and colleagues at the Universities of Urbino and Parma in Italy created URB597.
The study appears in an issue of Biological Psychiatry.

University of California

How to roll an Undercover joint

The Undercover Joint

What you will need:





1. Stick two rolling papers together to make a square with the glue strip along one side.

2. Take the cigarette and remove the filter by pinching the filter and rotating it with your finger and thumb.

3. Create a roach the same size as the filter to use in its place.

4. Cut the cigarette with a knife along the strip and remove the tobacco.

5. Roll the joint so it is the same size as the cigarette with the filter hanging out the end.

6. Secure the fake filter by using additional glue strips from rolling papers.

How to roll a Diamond joint

Diamond Joint

What you will need:

Rolling papers



  1. First you need to roll a longer then normal joint with a tight secure roach.
  2. Carefully cut the joint into two smaller joints using a razor blade or something sharp.
  3. Now roll two more joints just a little bit thinner the the first and don’t roll them with roaches.
  4.  When rolling these use less marijuana in them as well.
  5. Trim away any left over paper.
  6. Tear off the glue strips to attach the shorter joints to the the two long joints in a triangular shape of the diamond. Make sure it is tight.
  7. Carefully bend the two joints at their centers so that will create the diamond shape.
  8. Now bandage the other short joint to the free ends of the longer joints the same way we did it in step 5.

How to roll a Fatty


What you will need:

3 rolling papers



1. Fold one piece of the rolling paper in half with the glue facing out, lick the glue.

2. Now stick the other two papers together side by side slightly overlapping with the folded paper in between them.

3. Remove the folded piece from in between the other two pieces and press them back together.

4. Add your grinded up marijuana to the center of the paper and smoothen it length wise with your finger.

5. Start in the center and begin to fold the bottom edge over the mix with your index fingers.

6. Continue to do so until your fingers reach the corners of the joint.

7. Carefully replace your index fingers on the center of the joint and begin to roll it over once again and continue to the outside.

8. Use your saliva and use your lips to bring the glue down onto the joint.


How to roll a Cross Joint

Cross Joint

What you will need:

Rolling Papers

Index Cards



1. Create a roach using the index card about the length and diameter of the pen and seal it off with rolling papers.

2. Cut a circular hole on both sides of the roach about an inch from the end.

3. Make a second roach slightly narrower then the first and only a 1/3 the length.

4. Make a hole as big as you can in the middle of the second roach.

5. Place the second roach through the whole you just made in the first roach.

6. Spin the smaller roaches hole till they line up the with larger roaches holes.

7. Seal the roaches with glue strips from the rolling papers. Make sure its tight!

8. Add joints to each of the ends. And light the points simultaneously.

How to roll a tulip joint

What you will need:

Rolling Papers

Notecard/Index Card

Thread /String/Elastic Band



1. Roll your index card into the shape of a tube somewhat resembling a pen.

2. Take a few sheets of rolling paper and attach them together one on top of each other to make a square.

3. Fold the lower right corner of the square into the upper left hand corner right below the glue.

4. Apply saliva to the glue strip and fold it over onto itself and open the cone.

5. Begin to fill the cone with marijuana evenly and make sure to pack it tightly.

6. Now stick the stem into the center of the pocket of marijuana you just packed and pull the sides of the rolling paper around the stem.

7. Using your string or elastic band secure the tulip to the stem as tight as it will go.

8. Light the top and inhale from the bottom of the pen like tube.

How to roll a joint with a pen

What you need:




1. This is a pretty easy and basic step of rolling which I would recommend for most beginners.

2. Wrap your paper around the pen and lick the glue strip in sealing it around the pen.

3. Once you have created your cone from the pen you can begin to put your weed in the cone.

4. Then use the pen to lightly pack the weed into the cone

5. Finish packing then twist it off and spark away!

How to roll a joint with money

What you will need:

Dollar bill



1. Roll your dollar bill to the shape of a joint.

2. Pack your weed into the dollar bill.

3. Insert rolling paper into the bill making sure that the glue strip is facing up.

4. Fold the bill in half and roll upwards with your thumbs.

5. Now remove the joint remember to pinch the paper hard so it does not come apart.

6. Apply saliva to the glue and tighten up the joint.

7. It should now be ready to smoke!

Joint rolling for Beginners

What kind of papers to get?

There are a wide variety of papers out on the market and it would be easily accessible at your local smoke shop or head shop. If you cannot find any in your area you can always order wholesale packs of papers online which can also turn out being cheaper for you in the long run.

Remember when it comes down to rolling a joint you want the thinnest amount of paper between you and the marijuana. Organic hemp papers and rice papers are a very popular choice amongst smokers and have no hazardous glues or chemicals in their papers as other companies.

The art of rolling your basic joint

The mixture that you will be smoking is the most important aspect of the joint. You must make sure to grind up your marijuana to fine little pieces so that the mix can burn evenly throughout the joint

After your mix is grinded you want to place it evenly along the middle of the joint paper. Usually this will dictate the size of the joint. As a beginner you may use less of the mix to get the basis of rolling, then you can always stuff more after the joint is rolled.

Remember to add the roach or filter to the bottom tip of the rolling paper and roll the roach along with the mix all in one go.

Now to begin the rolling process this one is the hardest parts especially for beginners, as you have to have a very soft touch in order to not have the mix falling out as you attempt to roll. Using your two thumbs and index fingers pinch the mix together and gently roll the outside of the paper to keep the mix firm and even. When you feel the mix is tight and even, tuck down the facing paper edge with tips of your thumbs, wrap the excess paper around the joint, wet the glue strip with your tongue and seal it up against the other

The best joints are firm and not loose or too tight!

Potency Of Marijuana-Like Chemical In Body

A new means of improving the effects of anandamide – a natural, marijuana – like chemical in the body that provides pain relief has been discovered by UC Irvine and Italian researchers.

Led by Daniele Piomelli, UCI’s Louise Turner Arnold Chair in the Neurosciences, the team discovered an "escort" protein in brain cells that transports anandamide to sites within the cell where enzymes break it down. It was also found that blocking this protein – called FLAT – increases potency of anandamide.

Previous work by the researchers indicates that compounds that boost the natural abilities of anandamide may form the basis of pain medications that don’t produce sedation, addiction or other central nervous system side effects common with existing painkillers, such as opiates.

“These findings raise hope that the analgesic properties of marijuana can be harnessed for new, safe drugs,” said Piomelli, a professor of pharmacology. “Specific drug compounds we are creating that amplify the actions of natural, marijuana-like chemicals are showing great promise.”

Piomelli and his colleagues used computational methods for understanding how FLAT binds with anandamide and escorts it to cell sites to be degraded by fatty acid amide hydrolase (FAAH) enzymes.

Anandamide has been dubbed “the bliss molecule” for its similarities to the active ingredient in marijuana and is important in regulating food consumption.

It was speculated by Piomelli and his collaborators that FLAT (FAAH-like anandamide transporters) may be particularly useful in controlling certain forms of pain — that caused by damage to the central nervous system, for example — and curbing addiction to such drugs as nicotine and cocaine.

Researchers from UCI, Italy’s University of Parma and University of Bologna, and the Italian Institute of Technology participated in the study that was supported by grants from the U.S. National Institute on Drug Abuse, the U.S. National Institute on Alcohol Abuse & Alcoholism, and the U.S. National Institute of General Medical Sciences.

The study appeared in the Nov. 20 online version of Nature Neuroscience.